Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PDK1 (Ab-241). Bottom Image: Immunofluorescence staining of methanol-fixed MCF7 cells using PDK1 (Ab-241).Application
Western blot analysis of lysed extracts from MCF, 293 and PC12 cells using PDK1 (Ab-241).| Product Name | PDK1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDK1, PDPK2, PRO0461, KIAA0288, |
| Product description | Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-ζ, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development1) Scheid MP, et al. (2005) Mol Cell Biol; 25 (6): 2347-63Chen H, et al. (2001) Biochemistry; 40 (39): 11851-9Sato S, et al. (2002) J Biol Chem; 277 (42): 39360-7Lim MA, et al. (2003) Proc Natl Acad Sci U S A; 100 (24): 14006-11Beausoleil SA, et al. (2004) Proc Natl Acad Sci U S A; 101 (33): 12130-5 |
| Immunogen | PDK1 (Ab-241) antibody was raised against a peptide sequence around aa.239~243 (A-N-S-F-V) derived from Human PDK1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDPK1 |
|---|---|
| Alternative Names | PDK1 PDPK2 PRO0461 KIAA0288 |
| Molecular Weight(MW) | 63 kDa |
| Tissue Specificity | This antibody detects endogenous level of total PDK1 protein. |
| Entrez Gene | 5170 |
|---|---|
| Protein Accession | NP_002604.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PDK1 (Ab-241). Bottom Image: Immunofluorescence staining of methanol-fixed MCF7 cells using PDK1 (Ab-241).
Application
Western blot analysis of lysed extracts from MCF, 293 and PC12 cells using PDK1 (Ab-241).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
