Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using PDK1 (Phospho-Tyr9) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | PDK1 (phospho Tyr9) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | hPDK1, PDPK1, mPDK1, PkB kinase |
| Product description | Phosphorylates and activates not only PKB/AKT, but also PKA, PKC-zeta, RPS6KA1 and RPS6KB1. May play a general role in signaling processes and in development.1) Alessi D.R., Curr. Biol. 7:261-269(1997). |
| Immunogen | PDK1 (phospho Tyr9) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 9 (A-L-Y (p)-D-A) derived from Human PDK1. |
| Modification | p-Tyr9 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDPK1 |
|---|---|
| Alternative Names | hPDK1 PDPK1 mPDK1 PkB kinase |
| Molecular Weight(MW) | 63 kDa |
| Tissue Specificity | The antibody detects endogenous levels of PDK1 only when phosphorylated at tyrosine 9. |
| Entrez Gene | 5170 |
|---|---|
| Protein Accession | NP_002604.1 |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using PDK1 (Phospho-Tyr9) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
