Application
Flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded mouse heart tissue reacted with PEA-15 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of PEA-15 Antibody in T47D cell line lysates (35ug/lane)Application
Western blot analysis of PEA-15 Antibody in mouse lung tissue lysates (35ug/lane)| Product Name | PEA-15 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Astrocytic phosphoprotein PEA-15, 15 kDa phosphoprotein enriched in astrocytes, Phosphoprotein enriched in diabetes, PED, PEA15 |
| Product description | PEA15 is a death effector domain (DED)-containing protein predominantly expressed in the central nervous system, particularly in astrocytes.1) Trencia,A., et.al., Mol. Cell. Biol. 23 (13), 4511-4521 (2003) |
| Immunogen | This PEA-15 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-115 amino acids from the C-terminal region of human PEA-15. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PEA15 |
|---|---|
| Alternative Names | Astrocytic phosphoprotein PEA-15 15 kDa phosphoprotein enriched in astrocytes Phosphoprotein enriched in diabetes PED PEA15 |
| Molecular Weight(MW) | 15 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Hamster |
Application
Flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded mouse heart tissue reacted with PEA-15 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of PEA-15 Antibody in T47D cell line lysates (35ug/lane)
Application
Western blot analysis of PEA-15 Antibody in mouse lung tissue lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
