Application
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using PEK/PERK (Phospho-Thr981) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | PEK/PERK (phospho Thr981) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E2AK3, PERK, PEK, EIF2AK3 |
| Product description | Phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation and thus to a rapid reduction of translational initiation and repression of global protein synthesis. Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1)1) Shi Y., An J., J. Biol. Chem. 274:5723-5730(1999). |
| Immunogen | PEK/PERK (phospho Thr981) antibody was raised against a peptide sequence around phosphorylation site of threonine 981 (A-R-H (p)-T-G) derived from Human PEK/PERK. |
| Modification | p-Thr981 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF2AK3 |
|---|---|
| Alternative Names | E2AK3 PERK PEK EIF2AK3 |
| Molecular Weight(MW) | 125 kDa |
| Tissue Specificity | The antibody detects endogenous levels of PEK/PERK only when phosphorylated at threonine 981. |
| Entrez Gene | 9451 |
|---|---|
| Protein Accession | NP_004827.4 |
Application
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma tissue using PEK/PERK (Phospho-Thr981) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
