Prefoldin 1 is a heterohexameric chaperone protein which assists in the correct folding of other proteins. It binds specifically to cytosolic chaperonin and transfers target proteins. Prefoldin may function by selectively targeting nascent actin and tubulin chains pending their transfer to cytosolic chaperonin for final folding and/or assembly. It promotes folding in an environment in which there are many competing pathways for nonnative proteins.1) Cowan, N.J., et al., (1999) Nat. Struct. Biol. 6 (11), 990-991.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PFDN1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
PFDN1 antibody can be used for detection of PFDN1 by ELISA at 1:62500. PFDN1 antibody can be used for detection of PFDN1 by western blot at 5.0-8.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 at 5.0-8.0 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
PFDN1 antibody can be used for detection of PFDN1 by ELISA at 1:62500. PFDN1 antibody can be used for detection of PFDN1 by western blot at 5.0-8.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PFDN1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt