Application
Antibody is used in Western blot to detect PFKFB4 in mouse brain tissue lysate.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKFB4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | PFKFB4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4, 6PF-2-K/Fru-2,6-P2ase 4, PFK/FBPase 4, 6PF-2-K/Fru-2,6-P2ase testis-type isozyme, 6-phosphofructo-2-kinase, Fructose-2,6-bisphosphatase, PFKFB4 |
| Product description | Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK).1) Sakai, A., et al., J. Biochem. 119(3):506-511 (1996). Manzano, A., et al., Gene 229 (1-2), 83-89 (1999). |
| Immunogen | This PFKFB4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 266-296 amino acids from the Central region of human PFKFB4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | PFKFB4 |
|---|---|
| Alternative Names | 6-phosphofructo-2-kinase/fructose-2 6-bisphosphatase 4 6PF-2-K/Fru-2 6-P2ase 4 PFK/FBPase 4 6PF-2-K/Fru-2 6-P2ase testis-type isozyme 6-phosphofructo-2-kinase Fructose-2 6-bisphosphatase PFKFB4 |
| Molecular Weight(MW) | 54 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Antibody is used in Western blot to detect PFKFB4 in mouse brain tissue lysate.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PFKFB4 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
