Application
Antibody used in WB on Mouse at: 1:2000 (Lane 1: 20 ug murine non-pregnant uteria tissue, Lane 2: 20 ug murine non-laboring uterine tissue, Lane 3: 20 ug murine laboring uterine tissue, Lane 4: 20 ug murine preterm laboring uterine tissue).Application
Antibody used in WB on Human at: 1:2000 (Lane 1. 20 ug human pregnant uterine muscle cells + hormone Lane 2. 20 ug human pregnant uterine muscle cells - hormone).Application
Antibody used in WB on Human Liver at 0.2-1 ug/ml.| Product Name | PFN1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PFN1, ALS18 |
| Product description | PFN1 is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome.The protein encoded by this gene is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals. Deletion of this gene is associated with Miller-Dieker syndrome. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.1) Wen, K.K., (2008) J. Biol. Chem. 283 (14), 9444-9453. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PFN1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PFN1 antibody can be used for detection of PFN1 by ELISA at 1:12500. PFN1 antibody can be used for detection of PFN1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PFN1 |
|---|---|
| Alternative Names | PFN1 ALS18 |
| Molecular Weight(MW) | 15 kDa |
Application
Antibody used in WB on Mouse at: 1:2000 (Lane 1: 20 ug murine non-pregnant uteria tissue, Lane 2: 20 ug murine non-laboring uterine tissue, Lane 3: 20 ug murine laboring uterine tissue, Lane 4: 20 ug murine preterm laboring uterine tissue).
Application
Antibody used in WB on Human at: 1:2000 (Lane 1. 20 ug human pregnant uterine muscle cells + hormone Lane 2. 20 ug human pregnant uterine muscle cells - hormone).
Application
Antibody used in WB on Human Liver at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10409 - Fetal Liver Tissue Lysate |
|---|---|
| Application Notes | PFN1 antibody can be used for detection of PFN1 by ELISA at 1:12500. PFN1 antibody can be used for detection of PFN1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PFN1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
