Application
Immunocytochemistry of PHAP in Raji cells with PHAP antibody at 1 μg/mL.Application
Western blot analysis of PHAP expression in human Raji cell lysate with PHAP antibody at 1 μg/mL. The wide and strong band below PHAP III is a doublelet composed of PHAP I (upper) and PHAP I2a (lower).| Product Name | PHAP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PHAP Antibody: LANP, MAPM, PP32, HPPCn, PHAP1, PHAPI, I1PP2A, C15orf1, LANP, Acidic leucine-rich nuclear phosphoprotein 32 family member A, Acidic nuclear phosphoprotein pp32 |
| Product description | PHAP Antibody: Apoptosis is related to many diseases and development. Caspase-9 plays a central role in cell death induced by a variety of apoptosis activators. Cytochrome c, after released from mitochondria, binds to Apaf-1, which forms an apoptosome that in turn binds to and activate procaspase-9. Activated caspase-9 cleaves and activates the effector caspases (caspase-3, -6 and -7), which are responsible for the proteolytic cleavage of many key proteins in apoptosis. The tumor suppressor putative HLA-DR-associated proteins (PHAPs) were recently identified as important regulators of mitochondrion apoptosis. PHAP appears to facilitate apoptosome-medicated caspase-9 activation and to stimulate the mitochondrial apoptotic pathway. PHAP was also shown to oppose both Ras- and Myc-medicated cell transformation.1) Jiang X, Kim HE, Shu H, Zhao Y, Zhang H, Kofron J, Donnelly J, Burns D, Ng SC , Rosenberg S, Wang X. Distinctive roles of PHAP proteins and prothymosin-α in a death regulatory pathway. Science. 2003;299(5604):223-6. |
| Immunogen | PHAP antibody was raised with a synthetic peptide corresponding to amino acids at amino terminus of human PHAP. The immunogen is located within the first 50 amino acids of PHAP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| PHAP antibody can be used for detection of all three identified isoforms of PHAP including PHAP I, PHAP I2a, and PHAP III by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL.: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ANP32A |
|---|---|
| Alternative Names | PHAP Antibody: LANP MAPM PP32 HPPCn PHAP1 PHAPI I1PP2A C15orf1 LANP Acidic leucine-rich nuclear phosphoprotein 32 family member A Acidic nuclear phosphoprotein pp32 |
| Sequence Similarities | 2) Cat. No. 17-007 - Raji Cell Slide |
Application
Immunocytochemistry of PHAP in Raji cells with PHAP antibody at 1 μg/mL.
Application
Western blot analysis of PHAP expression in human Raji cell lysate with PHAP antibody at 1 μg/mL. The wide and strong band below PHAP III is a doublelet composed of PHAP I (upper) and PHAP I2a (lower).| Positive Control | 1) Cat. No. 1207 - Raji Cell Lysate |
|---|---|
| Application Notes | PHAP antibody can be used for detection of all three identified isoforms of PHAP including PHAP I, PHAP I2a, and PHAP III by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PHAP antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PHAP Antibody is supplied in PBS containing 0.02% sodium azide. |
