Application
Antibody used in IHC on Human Stomach.Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Product Name | PHB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PHB, PHB1, HEL-215, HEL-S-54e |
| Product description | Prohibitin is from an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA.1) Fraering, P.C., et al., (2004) Biochemistry 43 (30), 9774-9789. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PHB. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| PHB antibody can be used for detection of PHB by ELISA at 1:1562500. PHB antibody can be used for detection of PHB by western blot at 1.0-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PHB |
|---|---|
| Alternative Names | PHB PHB1 HEL-215 HEL-S-54e |
| Molecular Weight(MW) | 30 kDa |
Application
Antibody used in IHC on Human Stomach.
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | PHB antibody can be used for detection of PHB by ELISA at 1:1562500. PHB antibody can be used for detection of PHB by western blot at 1.0-2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PHB antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
