Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Confocal immunofluorescent analysis of PHB Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).Application
Western blot analysis of PHB Antibody in Hela cell line lysates (35ug/lane)Application
Western blot analysis of PHB using rabbit polyclonal PHB Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PHB gene.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PHB antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | PHB Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Prohibitin, PHB |
| Product description | Prohibitin is an evolutionarily conserved protein that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region.1) Gregory-Bass,R.C., Int. J. Cancer 122 (9), 1923-1930 (2008) |
| Immunogen | This PHB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-117 amino acids from the Central region of human PHB. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For IHC-P starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PHB |
|---|---|
| Alternative Names | Prohibitin PHB |
| Molecular Weight(MW) | 30 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Confocal immunofluorescent analysis of PHB Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Application
Western blot analysis of PHB Antibody in Hela cell line lysates (35ug/lane)
Application
Western blot analysis of PHB using rabbit polyclonal PHB Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PHB gene.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PHB antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For IHC-P starting dilution is: 1:10~50 For WB starting dilution is: 1:1000 For IF starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
