Application
Antibody used in IHC on Human Stomach.Application
Antibody used in WB on Human 293T at 1.25 ug/ml.| Product Name | PHF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PHF1, PCL1, PHF2, hPHF1, MTF2L2, TDRD19C |
| Product description | PHF1 has significant sequence similarity with Drosophila Polycomblike. It contains a zinc finger-like PHD (plant homeodomain) finger which is distinct from other classes of zinc finger motifs and which shows the typical Cys4-His-Cys3 arrangement. PHD finger genes are thought to belong to a diverse group of transcriptional regulators possibly affecting eukaryotic gene expression by influencing chromatin structure. This gene encodes a protein with significant sequence similarity to Drosophila Polycomblike. The encoded protein contains a zinc finger-like PHD (plant homeodomain) finger which is distinct from other classes of zinc finger motifs and which shows the typical Cys4-His-Cys3 arrangement. PHD finger genes are thought to belong to a diverse group of transcriptional regulators possibly affecting eukaryotic gene expression by influencing chromatin structure. Two transcript variants have been found for this gene.1) Yang, X.J., (2001) J. Biol. Chem. 276 (46), 43065-43073. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PHF1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| PHF1 antibody can be used for detection of PHF1 by ELISA at 1:1562500. PHF1 antibody can be used for detection of PHF1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PHF1 |
|---|---|
| Alternative Names | PHF1 PCL1 PHF2 hPHF1 MTF2L2 TDRD19C |
| Molecular Weight(MW) | 62 kDa |
Application
Antibody used in IHC on Human Stomach.
Application
Antibody used in WB on Human 293T at 1.25 ug/ml.| Positive Control | 1) 293T Cell Lysate |
|---|---|
| Application Notes | PHF1 antibody can be used for detection of PHF1 by ELISA at 1:1562500. PHF1 antibody can be used for detection of PHF1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PHF1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
