Application
Antibody used in WB on Human Jurkat 5.0 ug/ml.Application
Antibody used in WB on Human, Mouse at 1:1000.| Product Name | PHF6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PHF6, BORJ, MGC14797, BFLS, CENP-31 |
| Product description | PHF6 is a member of the plant homeodomain (PHD)-like finger (PHF) family. PHF6 is a protein with two PHD-type zinc finger domains, indicating a potential role in transcriptional regulation that localizes to the nucleolus. Mutations affecting the coding region of its gene or the splicing of the transcript have been associated with Borjeson-Forssman-Lehmann syndrome (BFLS), a disorder characterized by mental retardation, epilepsy, hypogonadism, hypometabolism, obesity, swelling of subcutaneous tissue of the face, narrow palpebral fissures, and large ears.This gene is a member of the plant homeodomain (PHD)-like finger (PHF) family. It encodes a protein with two PHD-type zinc finger domains, indicating a potential role in transcriptional regulation, that localizes to the nucleolus. Mutations affecting the coding region of this gene or the splicing of the transcript have been associated with Borjeson-Forssman-Lehmann syndrome (BFLS), a disorder characterized by mental retardation, epilepsy, hypogonadism, hypometabolism, obesity, swelling of subcutaneous tissue of the face, narrow palpebral fissures, and large ears. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.1) Vallee, D., (2004) J. Med. Genet. 41 (10), 778-783. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PHF6. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PHF6 antibody can be used for detection of PHF6 by ELISA at 1:312500. PHF6 antibody can be used for detection of PHF6 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHuman |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PHF6 |
|---|---|
| Alternative Names | PHF6 BORJ MGC14797 BFLS CENP-31 |
| Molecular Weight(MW) | 41 kDa, 35 kDa |
Application
Antibody used in WB on Human Jurkat 5.0 ug/ml.
Application
Antibody used in WB on Human, Mouse at 1:1000.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | PHF6 antibody can be used for detection of PHF6 by ELISA at 1:312500. PHF6 antibody can be used for detection of PHF6 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PHF6 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
