Application
Immunohistochemistry of PIAS3 in human breast carcinoma tissue with PIAS3 antibody at 5 μg/mL.Application
Western blot analysis of PIAS3 in K562 cell lysate with PIAS3 antibody at 1 μg/mL.| Product Name | PIAS3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIAS3 Antibody: ZMIZ5, E3 SUMO-protein ligase PIAS3, Protein inhibitor of activated STAT protein 3 |
| Product description | PIAS3 Antibody: The PIAS (protein inhibitor of activated STAT) proteins play a crucial role as transcriptional coregulators in various cellular pathways, including the STAT, p53 and the steroid hormone signaling pathway. The PIAS protein family includes at least five evolutionarily conserved genes, including PIAS3. The major function of the PIAS proteins is the control of gene transcription and can also act as small ubiquitin-like-modifier (SUMO) E3 ligases. PIAS3 binds specifically to STAT3 following the stimulation of STAT3. Increased expression of PIAS3 has been observed in several human cancers, including lung, breast, and brain tumors, but not in anaplastic lymphoma kinase-positive T/null-cell lymphomas, indicating that PIAS3 plays multiple roles in different tissue and cell types.1) Shuai K and Liu B. Regulation of gene-activation pathways by PIAS proteins in the immune system. Nat. Rev. Immunol.2005; 5:593-605. |
| Immunogen | PIAS3 antibody was raised against a 12 amino acid synthetic peptide near the carboxy terminus of human PIAS3. The immunogen is located within amino acids 410 - 460 of PIAS3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PIAS3 antibody can be used for detection of PIAS3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PIAS3 |
|---|---|
| Alternative Names | PIAS3 Antibody: ZMIZ5 E3 SUMO-protein ligase PIAS3 Protein inhibitor of activated STAT protein 3 |
Application
Immunohistochemistry of PIAS3 in human breast carcinoma tissue with PIAS3 antibody at 5 μg/mL.
Application
Western blot analysis of PIAS3 in K562 cell lysate with PIAS3 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1204 - K562 Cell Lysate |
|---|---|
| Application Notes | PIAS3 antibody can be used for detection of PIAS3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PIAS3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PIAS3 Antibody is supplied in PBS containing 0.02% sodium azide. |
