Application
Immunocytochemistry staining of HeLa using PID antibody at 10 μg/mL.Application
Immunohistochemistry of PID in human small intestine tissue with PID antibody at 2.5 μg/ml.Application
Immunofluorescence of PID in HeLa cells with PID antibody at 10 ug/mL.Application
Western blot analysis of PID expression in HeLa whole cell lysates in the absence (A) or presence (B) of blocking peptide with PID antibody at 1 μg/mL.| Product Name | PID Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PID Antibody: PID, MTA1L1, PID, Metastasis-associated protein MTA2, Metastasis-associated 1-like 1, MTA1-L1 protein |
| Product description | PID Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 pathway, including Chk2, p53R2, p53AIP1, Noxa, PIDD, and PID/MTA2, were recently discovered. The transcriptional activity of p53 is modulated by protein stability and acetylation. PID/MTA2, also termed MTA1-L1, was found to be a subunit of nucleosome remodeling and deacetylating (NRD/NuRD) complex. PID/MTA2 modulates the enzymatic activity of the histone deacetylase complex and its expression reduces the levels of acetylated p53. Deacetylation of p53 by PID/MTA2 represses p53-dependent transcriptional activation and modulates p53-mediated cell growth arrest and apoptosis. PID/MTA2 is ubiquitously expressed in human tissues.1) Matsuoka S, Huang M, and Elledge SJ. Linkage of ATM to cell cycle regulation by the Chk2 protein kinase. Science 1998; 282:1893-7. |
| Immunogen | PID antibody was raised against a 17 amino acid peptide near the carboxy terminus of human PID. The immunogen is located within the last 50 amino acids of PID. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFIHC-PWB |
| PID antibody can be used for detection of PID by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and Immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MTA2 |
|---|---|
| Alternative Names | PID Antibody: PID MTA1L1 PID Metastasis-associated protein MTA2 Metastasis-associated 1-like 1 MTA1-L1 protein |
| Molecular Weight(MW) | Predicted: 73 kDa Observed: 75 kDa |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | PID antibody is predicted to not cross-react with MTA2 |
Application
Immunocytochemistry staining of HeLa using PID antibody at 10 μg/mL.
Application
Immunohistochemistry of PID in human small intestine tissue with PID antibody at 2.5 μg/ml.
Application
Immunofluorescence of PID in HeLa cells with PID antibody at 10 ug/mL.
Application
Western blot analysis of PID expression in HeLa whole cell lysates in the absence (A) or presence (B) of blocking peptide with PID antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | PID antibody can be used for detection of PID by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL and Immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PID antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PID Antibody is supplied in PBS containing 0.02% sodium azide. |
