PIK3R3 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1.1) Kallin, A., (2007) J. Lipid Res. 48 (7), 1628-1636.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PIK3R3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
PIK3R3 antibody can be used for detection of PIK3R3 by ELISA at 1:1562500. PIK3R3 antibody can be used for detection of PIK3R3 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Transfected 293T at 5.0 ug/ml.
Positive Control
1) Tranfected 293T Cell Lysate
Application Notes
PIK3R3 antibody can be used for detection of PIK3R3 by ELISA at 1:1562500. PIK3R3 antibody can be used for detection of PIK3R3 by western blot at 5.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PIK3R3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt