Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PIK3R3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of lysate from MDA-MB-453 cell line, using PI3KR3 Antibody (G331) RB1719 was diluted at 1:1000 at each lane.Application
Fluorescent image of U251 cells stained with PIK3R3 Antibody . Antibody was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).| Product Name | PIK3R3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Phosphatidylinositol 3-kinase regulatory subunit gamma, PI3-kinase regulatory subunit gamma, PI3K regulatory subunit gamma, PtdIns-3-kinase regulatory subunit gamma, Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma, PI3-kinase subunit p55-gamm |
| Product description | PIK3R3 binds to activated (phosphorylated) protein-tyrosine kinases through its SH2 domain and regulates their kinase activity. During insulin stimulation, it also binds to IRS-1. It is a component of a heterodimer of p110 (catalytic) and p55 (regulatory) subunits. The protein is expressed at highest levels in brain and testis. Lower levels are detected in adipose tissue, kidney, heart, lung and skeletal muscle. The protein contains 2 SH2 domains.1) Dey, B.R., et al., Gene 209 (1-2), 175-183 (1998). |
| Immunogen | This PIK3R3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 316-346 amino acids from the C-terminal region of human PIK3R3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For IF starting dilution is: 1:100 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PIK3R3 |
|---|---|
| Alternative Names | Phosphatidylinositol 3-kinase regulatory subunit gamma PI3-kinase regulatory subunit gamma PI3K regulatory subunit gamma PtdIns-3-kinase regulatory subunit gamma Phosphatidylinositol 3-kinase 55 kDa regulatory subunit gamma PI3-kinase subunit p55-gamm |
| Molecular Weight(MW) | 54 kDa |
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PIK3R3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of lysate from MDA-MB-453 cell line, using PI3KR3 Antibody (G331) RB1719 was diluted at 1:1000 at each lane.
Application
Fluorescent image of U251 cells stained with PIK3R3 Antibody . Antibody was diluted at 1:100 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).| Application Notes | For IF starting dilution is: 1:100 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
