Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PIM1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of PIM1 using PIM1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIM1 gene (Lane 2).| Product Name | PIM1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Serine/threonine-protein kinase pim-1, PIM1 |
| Product description | PIM1, which belongs to the Serine/Threonine protein kinase family, is thought to play a role in signal transduction in blood cells. The protooncogene PIM1 encodes a protein kinase upregulated in prostate cancer. It may affect the structure or silencing of chromatin by phosphorylating HP1 gamma/CBX3. PIM1 is expressed primarily in cells of the hematopoietic and germ line lineages.1) Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002). Pasqualucci, L., et al., Nature 412(6844):341-346 (2001). Koike, N., et al., FEBS Lett. 467(1):17-21 (2000). Reeves, R., et al., Gene 90(2):303-307 (1990). Telerman, A., et al., Mol. Cell. Biol. 8(4):1498-1503 (1988). |
| Immunogen | This PIM1 antibody is generated from rabbits immunized with PIM1 recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PIM1 |
|---|---|
| Alternative Names | Serine/threonine-protein kinase pim-1 PIM1 |
| Molecular Weight(MW) | 45 kDa |
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with PIM1 antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of PIM1 using PIM1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIM1 gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
