Application
Western blot analysis of PIP5K3 using rabbit polyclonal PIP5K3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIP5K3 gene (Lane 2).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | PIP5K3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 1-phosphatidylinositol 3-phosphate 5-kinase, Phosphatidylinositol 3-phosphate 5-kinase, FYVE finger-containing phosphoinositide kinase, PIKfyve, Phosphatidylinositol 3-phosphate 5-kinase type III, PIPkin-III, Type III PIP kinase, PIKFYVE, KIAA0981, PIP5K3 |
| Product description | PIP5K3 belongs to a large family of lipid kinases that alter the phosphorylation status of intracellular phosphatidylinositol. Signaling by phosphorylated species of phosphatidylinositol regulates diverse cellular processes, including membrane trafficking and cytoskeletal reorganization.1) Sbrissa, D., et al., Mol. Cell. Biol. 24(23):10437-10447 (2004). |
| Immunogen | This PIP5K3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1531-1561 amino acids from the Central region of human PIP5K3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PIKFYVE |
|---|---|
| Alternative Names | 1-phosphatidylinositol 3-phosphate 5-kinase Phosphatidylinositol 3-phosphate 5-kinase FYVE finger-containing phosphoinositide kinase PIKfyve Phosphatidylinositol 3-phosphate 5-kinase type III PIPkin-III Type III PIP kinase PIKFYVE KIAA0981 PIP5K3 |
| Molecular Weight(MW) | 237 kDa |
Application
Western blot analysis of PIP5K3 using rabbit polyclonal PIP5K3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PIP5K3 gene (Lane 2).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
