Application
Immunohistochemistry of PIST in rat colon tissue with PIST antibody at 1 μg/mL.Application
Western blot analysis of PIST in PC-3 cell lysate with PIST antibody at (A) 1, (B) 2 and (C) 4 μg/mL.Application
Immunofluorescence of PIST in Rat Colon cells with PIST antibody at 20 μg/mL.| Product Name | PIST Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIST Antibody: CAL, FIG, PIST, GOPC1, dJ94G16.2, CAL, Golgi-associated PDZ and coiled-coil motif-containing protein, CFTR-associated ligand |
| Product description | PIST Antibody: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components and is negatively regulated by TOR (Target of rapamycin). PIST, a PDZ-containing protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6. Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.1) Gozuacik D and Kimchi A. Autophagy as a cell death and tumor suppressor mechanism. Oncogene. 2004; 23:2891-906. |
| Immunogen | PIST antibody was raised against a 17 amino acid synthetic peptide from near the center of human PIST. The immunogen is located within amino acids 240 - 290 of PIST. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PIST antibody can be used for the detection of PIST by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | GOPC |
|---|---|
| Alternative Names | PIST Antibody: CAL FIG PIST GOPC1 dJ94G16.2 CAL Golgi-associated PDZ and coiled-coil motif-containing protein CFTR-associated ligand |
| Sequence Similarities | 2) Cat. No. 1472 - Rat Colon Tissue Lysate |
Application
Immunohistochemistry of PIST in rat colon tissue with PIST antibody at 1 μg/mL.
Application
Western blot analysis of PIST in PC-3 cell lysate with PIST antibody at (A) 1, (B) 2 and (C) 4 μg/mL.
Application
Immunofluorescence of PIST in Rat Colon cells with PIST antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1216 - PC-3 Cell Lysate |
|---|---|
| Application Notes | PIST antibody can be used for the detection of PIST by Western blot at 1 - 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 1 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PIST antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PIST Antibody is supplied in PBS containing 0.02% sodium azide. |
