Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKA alpha/beta CAT (Phospho-Thr197) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Immunofluorescence staining of methanol-fixed A549 cells using PKA alpha/beta CAT (Phospho-Thr197) Antibody.| Product Name | PKA alpha/beta CAT (phospho Thr197) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PRKACA , PRKACB, PKACA |
| Product description | Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts.1) Ghislaine Garrel, Endocrinology, Jun 1997; 138: 2259. |
| Immunogen | PKA alpha/beta CAT (phospho Thr197) antibody was raised against a peptide sequence around phosphorylation site of threonine 197 (R-T-W (p)-T-L) derived from Human PKA alpha/beta CAT. |
| Modification | p-Thr197 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IFIHC |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKACA |
|---|---|
| Alternative Names | PRKACA PRKACB PKACA |
| Molecular Weight(MW) | 40 kDa |
| Tissue Specificity | The antibody detects endogenous levels of PKA alpha/beta CAT only when phosphorylated at threonine 197. |
| Entrez Gene | 5566 |
|---|---|
| Protein Accession | NP_002721.1 |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PKA alpha/beta CAT (Phospho-Thr197) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Immunofluorescence staining of methanol-fixed A549 cells using PKA alpha/beta CAT (Phospho-Thr197) Antibody.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM288182