Application
Western blot analysis of extracts from COLO205 cells using PKC alpha (Phospho-Tyr658) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | PKC alpha (phospho Tyr658) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PKC-alpha, KPCA, PKC III, PKC-A |
| Product description | Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that is involved in positive and negative regulation of cell proliferation, apoptosis, differentiation, migration and adhesion, tumorigenesis, cardiac hypertrophy, angiogenesis, platelet function and inflammation, by directly phosphorylating targets such as RAF1, BCL2, CSPG4, TNNT2/CTNT, or activating signaling cascade involving MAPK1/3 (ERK1/2) and RAP1GAP. Involved in cell proliferation and cell growth arrest by positive and negative regulation of the cell cycle. Can promote cell growth by phosphorylating and activating RAF1, which mediates the activation of the MAPK/ERK signaling cascade, and/or by up-regulating CDKN1A, which facilitates active cyclin-dependent kinase (CDK) complex formation in glioma cells.1) Finkenzeller G., Nucleic Acids Res. 18:2183-2183(1990). |
| Immunogen | PKC alpha (phospho Tyr658) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 658 (F-S-Y (p)-V-N) derived from Human PKC alpha. |
| Modification | p-Tyr658 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKCA |
|---|---|
| Alternative Names | PKC-alpha KPCA PKC III PKC-A |
| Molecular Weight(MW) | 80 kDa |
| Tissue Specificity | The antibody detects endogenous levels of PKC alpha only when phosphorylated at tyrosine 658. |
| Entrez Gene | 5578 |
|---|---|
| Protein Accession | NP_002728.1 |
Application
Western blot analysis of extracts from COLO205 cells using PKC alpha (Phospho-Tyr658) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
