Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of PKC delta using rabbit polyclonal PKC delta Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCD gene (Lane 2).| Product Name | PKC delta Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein kinase C delta type, Tyrosine-protein kinase PRKCD, nPKC-delta, Protein kinase C delta type regulatory subunit, Protein kinase C delta type catalytic subunit, Sphingosine-dependent protein kinase-1, SDK1, PRKCD |
| Product description | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC delta is one of the PKC family members, and may play a role in antigen-dependent control of B-cell function.1) Nomoto, S., et al., Genes Cells 2(10):601-614 (1997). |
| Immunogen | This PKC delta antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-326 amino acids from the N-terminal region of human PKC delta. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PRKCD |
|---|---|
| Alternative Names | Protein kinase C delta type Tyrosine-protein kinase PRKCD nPKC-delta Protein kinase C delta type regulatory subunit Protein kinase C delta type catalytic subunit Sphingosine-dependent protein kinase-1 SDK1 PRKCD |
| Molecular Weight(MW) | 78 kDa |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of PKC delta using rabbit polyclonal PKC delta Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCD gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
