Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of PRKCE using rabbit polyclonal PKC epsilon Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCE gene (Lane 2).Application
Western blot analysis of anti-PKCepsilon Pab in placenta lysate. PKCepsilon was detected using purified Pab.| Product Name | PKC epsilon Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein kinase C epsilon type, nPKC-epsilon, PRKCE, PKCE |
| Product description | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. PKC epsilon is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior.1) Gray, M.O., et al., J. Biol. Chem. 279(5):3596-3604 (2004). |
| Immunogen | This PKC epsilon antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 206-236 amino acids from the N-terminal region of human PKC epsilon. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PRKCE |
|---|---|
| Alternative Names | Protein kinase C epsilon type nPKC-epsilon PRKCE PKCE |
| Molecular Weight(MW) | 84 kDa |
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of PRKCE using rabbit polyclonal PKC epsilon Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PRKCE gene (Lane 2).
Application
Western blot analysis of anti-PKCepsilon Pab in placenta lysate. PKCepsilon was detected using purified Pab.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
