Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PKC gamma antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of anti-PKC gamma C-term Pab in mouse brain lysate. PKC gamma was detected using purified Pab.| Product Name | PKC gamma Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein kinase C gamma type, PKC-gamma, PRKCG, PKCG |
| Product description | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC gamma is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14).1) Uchino, M., et al., J. Biol. Chem. 279(3):2254-2261 (2004). |
| Immunogen | This PKC gamma antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 305-336 amino acids from the C-terminal region of human PKC gamma. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | PRKCG |
|---|---|
| Alternative Names | Protein kinase C gamma type PKC-gamma PRKCG PKCG |
| Molecular Weight(MW) | 78 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PKC gamma antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of anti-PKC gamma C-term Pab in mouse brain lysate. PKC gamma was detected using purified Pab.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
