Application
Western blot analysis of anti-PKC mu N-term Pab in mouse kidney lysateApplication
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.| Product Name | PKC mu Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Serine/threonine-protein kinase D1, Protein kinase C mu type, Protein kinase D, nPKC-D1, nPKC-mu, PRKD1, PKD, PKD1, PRKCM |
| Product description | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. PKC mu is one of the PKC family members. The protein is a cytosolic serine-threonine kinase that binds to the trans-Golgi network and regulates the fission of transport carriers specifically destined to the cell surface.1) Rao, P.S., et al., Biochem. Biophys. Res. Commun. 310(3):1032-1038 (2003). |
| Immunogen | This PKC mu antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 361-389 amino acids from the N-terminal region of human PKC mu. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Gene Symbol | PRKD1 |
|---|---|
| Alternative Names | Serine/threonine-protein kinase D1 Protein kinase C mu type Protein kinase D nPKC-D1 nPKC-mu PRKD1 PKD PKD1 PRKCM |
| Molecular Weight(MW) | 102 kDa |
Application
Western blot analysis of anti-PKC mu N-term Pab in mouse kidney lysate
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma; HC = hepatocarcinoma.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
