Application
Western blot analysis of extracts from COS7 cells treated with PMA using Phospho-PKC zeta (Thr560) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | PKC zeta (phospho Thr560) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KPCZ, PKC-zeta, PKC2, PRKCZ, nPKC-zeta |
| Product description | This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.1) Nakayama M, et al. (2013)Nat Cell Biol 15, 249-60. Mayanglambam A, Bhavanasi D, Vijayan KV, Kunapuli SP (2011)Biochem Pharmacol 82, 505-13. Dettori R, et al. (2009)J Biol Chem 284, 30318-27. |
| Immunogen | PKC zeta (phospho Thr560) antibody was raised against a peptide sequence around phospho rylation site of threonine560 (Q-L-T (p)-P-D) derived from Human PKC zeta . |
| Modification | p-Thr560 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKCZ |
|---|---|
| Alternative Names | KPCZ PKC-zeta PKC2 PRKCZ nPKC-zeta |
| Molecular Weight(MW) | 80 kDa |
| Tissue Specificity | The antibody detects endogenous level of PKC zeta only when phosphorylated at threonine 560. |
| Entrez Gene | 5590 |
|---|---|
| Protein Accession | NP_001028753.1 |
Application
Western blot analysis of extracts from COS7 cells treated with PMA using Phospho-PKC zeta (Thr560) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
