Application
Western blot analysis of extracts from HeLa cells treated with Heatshock using Phospho-PKCB (Ser661) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | PKCB (phospho Ser661) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PIM-1, EC 2.7.11.1 |
| Product description | Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1.1) Zhu M, Chen J, Jiang H, Miao C (2013) Cardiovasc Diabetol 12, 13 |
| Immunogen | PKCB (phospho Ser661) antibody was raised against a peptide sequence around phosphorylation site of serine 661 (G-F-S (p)-Y-T) derived from Human PKCB. |
| Modification | p-Ser661 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKCB |
|---|---|
| Alternative Names | PIM-1 EC 2.7.11.1 |
| Molecular Weight(MW) | 77 kDa |
| Tissue Specificity | The antibody detects endogenous level of PKCB only when phosphorylated at serine 661. |
| Entrez Gene | 5579 |
|---|---|
| Protein Accession | NP_002729.2 |
Application
Western blot analysis of extracts from HeLa cells treated with Heatshock using Phospho-PKCB (Ser661) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
