Application
Western blot analysis of extracts from HepG2 cells treated with PMA using Phospho-PKCD (Tyr64) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | PKCD (phospho Tyr64) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KPCD, PKC-delta, PRKCD, kinase PKC-delta, nPKC-delta |
| Product description | Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that plays contrasting roles in cell death and cell survival by functioning as a pro-apoptotic protein during DNA damage-induced apoptosis, but acting as an anti-apoptotic protein during cytokine receptor-initiated cell death, is involved in tumor suppression as well as survival of several cancers, is required for oxygen radical production by NADPH oxidase and acts as positive or negative regulator in platelet functional responses.1) Wie SM, et al. (2014)J Biol Chem. Adwan TS, et al. (2011)J Biol Chem 286, 35716-24. Lomonaco SL, et al. (2008) J Biol Chem 283, 17731-9. |
| Immunogen | PKCD (phospho Tyr64) antibody was raised against a peptide sequence around phosphorylation site of Tyrosine 64 (H-I-Y (p)-E-G) derived from Human PKCD. |
| Modification | p-Tyr64 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKCD |
|---|---|
| Alternative Names | KPCD PKC-delta PRKCD kinase PKC-delta nPKC-delta |
| Molecular Weight(MW) | 78 kDa |
| Tissue Specificity | The antibody detects endogenous level of PKCD only when phosphorylated at tyrosine 64. |
| Entrez Gene | 5580 |
|---|---|
| Protein Accession | NP_006245.2 |
Application
Western blot analysis of extracts from HepG2 cells treated with PMA using Phospho-PKCD (Tyr64) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
