Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKCδ (Ab-645). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using PKCδ (Ab-645).Application
Western blot analysis of lysed extracts from PC12 cells using PKCδ (Ab-645).| Product Name | PKCdelta Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MAY1, PKCD, CVID9, nPKC-delta, G0S7 |
| Product description | This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol. which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.1) Kei Sakamoto, et, al. (2003) Am J Physiol Endocrinol Metab, ; 285: E1081 - E1088. Ling Zhang, et, al. (2004) J. Biol. Chem ; 279: 28315 - 28319. Kristof Van Kolen et, al. (2006) FEBS J ; 273: 1843 - 1854. Martin Villalba, et, al. (2002) J. Cell Biol ; 157: 253. |
| Immunogen | PKCdelta (Ab-645) antibody was raised against a peptide sequence around aa. 643~647 (R-L-S-Y-S) derived from Human PKCδ. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKCD |
|---|---|
| Alternative Names | MAY1 PKCD CVID9 nPKC-delta G0S7 |
| Molecular Weight(MW) | 78 kDa |
| Tissue Specificity | This antibody detects endogenous level of total PKCδ protein. |
| Entrez Gene | 5580 |
|---|---|
| Protein Accession | NP_006245.2 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using PKCδ (Ab-645). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using PKCδ (Ab-645).
Application
Western blot analysis of lysed extracts from PC12 cells using PKCδ (Ab-645).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
