Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCzeta (Ab-410).Application
Western blot analysis of extract from 293 cells and HeLa using PKCzeta (Ab-410).| Product Name | PKCzeta Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PKC2, PKC-ZETA, GAD65, PKC2, PRKCZ |
| Product description | This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.1) Parmentier JH, et al. (2004) BMC Cell Biol; 5: 4. Castrillo A, et al. (2003) Mol Cell Biol; 23 (4): 1196-1208. Paramio JM, et al. ( 2001) Mol Cell Biol; 21 (21): 7449-7459. Braiman L, et al. (2001) Mol Cell Biol; 21 (22): 7852-7861. |
| Immunogen | PKCzeta (Ab-410) antibody was raised against a peptide sequence around aa. 408~412 ( T-S-T-F-C) derived from PKCzeta . |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PRKCZ |
|---|---|
| Alternative Names | PKC2 PKC-ZETA GAD65 PKC2 PRKCZ |
| Molecular Weight(MW) | 78 kDa |
| Tissue Specificity | This antibody detects endogenous levels of total PKCzeta protein. |
| Entrez Gene | 5590 |
|---|---|
| Protein Accession | NP_002735.3 |
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCzeta (Ab-410).
Application
Western blot analysis of extract from 293 cells and HeLa using PKCzeta (Ab-410).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
