Application
Western blot analysis of in mouse testis tissue lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of in Hela cell line lysates (35ug/lane)| Product Name | PKM1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Pyruvate kinase PKM, Cytosolic thyroid hormone-binding protein, CTHBP, Opa-interacting protein 3, OIP-3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, THBP1, Tumor M2-PK, p58, PKM, OIP3, PK2, PK3, PKM2 |
| Product description | PKM1 is a pyruvate kinase that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate to ADP, generating ATP and pyruvate. This protein has been shown to interact with thyroid hormone and may mediate cellular metabolic effects induced by thyroid hormones. The protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis.1) Dombrauckas J.D.Biochemistry 44:9417-9429(2005) Daub H., Olsen J.V.Mol. Cell 31:438-448(2008) Ashizawa K., McPhie P.Biochemistry 30:7105-7111(1991) |
| Immunogen | This PKM1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 383-417 amino acids from the C-terminal region of human PKM1. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~200: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PKM |
|---|---|
| Alternative Names | Pyruvate kinase PKM Cytosolic thyroid hormone-binding protein CTHBP Opa-interacting protein 3 OIP-3 Pyruvate kinase 2/3 Pyruvate kinase muscle isozyme Thyroid hormone-binding protein 1 THBP1 Tumor M2-PK p58 PKM OIP3 PK2 PK3 PKM2 |
| Molecular Weight(MW) | 58 kDa |
Application
Western blot analysis of in mouse testis tissue lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of in Hela cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
