Application
Western blot analysis of lysed extracts from HepG2 and 293 cells using PKM1/2 Antibody.| Product Name | PKM2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PK3, TCB, OIP3, PKM2, CTHBP, THBP1, HEL-S-30, CAGH44, TNRC10, PKM, PK3, PK2 |
| Product description | Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.1) Christofk, H. R. et al. (2008) Nature 452, 230-3. Mazurek, S. et al. (2005) Semin Cancer Biol 15, 300-8. Dombrauckas, J. D. et al. (2005) Biochemistry 44, 9417-29. Hitosugi, T. et al. (2009) Sci Signal 2, ra73. |
| Immunogen | PKM2 antibody was raised against a peptide sequence around aa.405~409 (T-S-D-P-T) derived from Human PKM2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PKM |
|---|---|
| Alternative Names | PK3 TCB OIP3 PKM2 CTHBP THBP1 HEL-S-30 CAGH44 TNRC10 PKM PK3 PK2 |
| Molecular Weight(MW) | 60 kDa |
| Tissue Specificity | This antibody detects endogenous levels of total PKM2 protein. |
| Entrez Gene | 5315 |
|---|---|
| Protein Accession | NP_872270.1 |
Application
Western blot analysis of lysed extracts from HepG2 and 293 cells using PKM1/2 Antibody.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM288223