PLAG1 is a zinc finger protein with 2 putative nuclear localization signals. PLAG1, which is developmentally regulated, has been shown to be consistently rearranged in pleomorphic adenomas of the salivary glands. PLAG1 is activated by the reciprocal chromosomal translocations involving 8q12 in a subset of salivary gland pleomorphic adenomas. Pleomorphic adenoma gene 1 encodes a zinc finger protein with 2 putative nuclear localization signals. PLAG1, which is developmentally regulated, has been shown to be consistently rearranged in pleomorphic adenomas of the salivary glands. PLAG1 is activated by the reciprocal chromosomal translocations involving 8q12 in a subset of salivary gland pleomorphic adenomas. Three transcript variants encoding two different isoforms have been found for this gene.1) Persson, F., (2008) Oncogene 27 (21), 3072-3080.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PLAG1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
PLAG1 antibody can be used for detection of PLAG1 by ELISA at 1:312500. PLAG1 antibody can be used for detection of PLAG1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Hum. Fetal Muscle at 1 ug/ml.
Application
Antibody used in WB on Hum. Fetal Lung at 1 ug/ml.
Application
Antibody used in WB on Human OVCAR-3 at 0.2-1 ug/ml.
Positive Control
1) OVCAR-3 Cell Lysate
Application Notes
PLAG1 antibody can be used for detection of PLAG1 by ELISA at 1:312500. PLAG1 antibody can be used for detection of PLAG1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PLAG1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.