Application
Immunofluorescence staining of methanol-fixed HeLa cells using PLC-γ1 (phospho-Tyr771).Application
Western blot analysis of lysed extracts from 3T3 cells untreated or treated with serum using PLC-γ1 (phospho-Tyr771).| Product Name | PLC gamma1 (phospho Tyr771) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PLC1, NCKAP3, PLC-II, PLC148, PLCgamma1, CTNNB, OK/SW-cl.35, PRO2286, Phosphoinositide phospholipase C, Phospholipase C-gamma-1 |
| Product description | PLC-gamma is a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase.1) Yue, C. et al. (1998) J. Biol. Chem. 273, 18023-18027. Margolis, B. et al. (1989) Cell 57, 1101-1107. Yue, C. et al. (2000) J. Biol. Chem. 275, 30220-30225. |
| Immunogen | PLC-gamma1 (phospho-Tyr771) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 771 (P-D-Y (p) -G-A) derived from Human PLC-γ1. |
| Modification | p-Tyr771 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFWB |
| Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLCG1 |
|---|---|
| Alternative Names | PLC1 NCKAP3 PLC-II PLC148 PLCgamma1 CTNNB OK/SW-cl.35 PRO2286 Phosphoinositide phospholipase C Phospholipase C-gamma-1 |
| Molecular Weight(MW) | 155 kDa |
| Tissue Specificity | This antibody detects endogenous level of PLC-gamma1 only when phosphorylated at tyrosine 771. |
| Entrez Gene | 5335 |
|---|---|
| Protein Accession | NP_002651.2 |
Application
Immunofluorescence staining of methanol-fixed HeLa cells using PLC-γ1 (phospho-Tyr771).
Application
Western blot analysis of lysed extracts from 3T3 cells untreated or treated with serum using PLC-γ1 (phospho-Tyr771).| Application Notes | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
