Application
Immunofluorescence staining of methanol-fixed HeLa cells using PLC-γ2 (Ab-1217).Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PLC-γ2 (Ab-1217).| Product Name | PLC gamma2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FCAS3, APLAID, PLC-IV, PLC-gamma-2, Afh, Fbl3a, Fbxl3a, Ovtm, PLC-gamma2, Phospholipase C-gamma-2 |
| Product description | The production of the second messenger molecules diacylglycerol. (DAG) and inositol 1, 4, 5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.1) Singer, W. D. et al. (1997) Annu. Rev. Biochem. 66, 475-509. Smrcka, A. V. et al. (1991) Science 251, 804-807. Yue, C. et al. (2000) J. Biol. Chem. 275, 30220-30225. Watanabe, D. et al. (2001) J. Biol. Chem. 276, 38595-38601. |
| Immunogen | PLC-gamma2 (Ab-1217) antibody was raised against a peptide sequence around aa.1215~1219 (F-L-Y-D-T) derived from Human PLCγ2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHC |
| Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLCG2 |
|---|---|
| Alternative Names | FCAS3 APLAID PLC-IV PLC-gamma-2 Afh Fbl3a Fbxl3a Ovtm PLC-gamma2 Phospholipase C-gamma-2 |
| Molecular Weight(MW) | 150 kDa |
| Tissue Specificity | This antibody detects endogenous level of total PLC-γ2 protein. |
| Entrez Gene | 5336 |
|---|---|
| Protein Accession | NP_002652.2 |
Application
Immunofluorescence staining of methanol-fixed HeLa cells using PLC-γ2 (Ab-1217).
Application
Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PLC-γ2 (Ab-1217).| Application Notes | Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
