Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of PLCB1 Antibody in HL-60 cell line lysates (35ug/lane)| Product Name | PLCB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-1, PLC-154, Phosphoinositide phospholipase C-beta-1, Phospholipase C-I, PLC-I, Phospholipase C-beta-1, PLC-beta-1, PLCB1, KIAA0581 |
| Product description | The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits, alpha-q and alpha-11.1) Need, A.C., et al. Hum. Mol. Genet. 18(23):4650-4661(2009) |
| Immunogen | This PLCB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1148-1176 amino acids from the C-terminal region of human PLCB1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PLCB1 |
|---|---|
| Alternative Names | 1-phosphatidylinositol 4 5-bisphosphate phosphodiesterase beta-1 PLC-154 Phosphoinositide phospholipase C-beta-1 Phospholipase C-I PLC-I Phospholipase C-beta-1 PLC-beta-1 PLCB1 KIAA0581 |
| Molecular Weight(MW) | 139 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of PLCB1 Antibody in HL-60 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
