Application
Immunofluorescence staining of methanol-fixed HeLa cells using PLCγ2 (Ab-753).Application
Western blot analysis of lysed extracts from 293 and 3T3 cells using PLCγ2 (Ab-753).| Product Name | PLCgamma2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | FCAS3, APLAID, PLC-IV, PLC-gamma-2, Phosphoinositide phospholipase C, Phospholipase C-gamma-2 |
| Product description | The production of the second messenger molecules diacylglycerol. (DAG) and inositol 1, 4, 5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. It is a crucial enzyme in transmembrane signaling.1) Kim YJ, et al. (2004) Mol Cell Biol 24: 9986-9999Humphries LA, et al. (2004) J Biol Chem 279 : 37651-37661Suzuki-Inoue K, et al. (2004) Biochem J 378 : 1023-1029Rodriguez R, et al. (2003) Biochem J 374 : 269-280 |
| Immunogen | PLCgamma2 (Ab-753) antibody was raised against a peptide sequence around aa. 751~755 (S-L-Y-D-V) derived from Human PLCγ2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFWB |
| Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLCG2 |
|---|---|
| Alternative Names | FCAS3 APLAID PLC-IV PLC-gamma-2 Phosphoinositide phospholipase C Phospholipase C-gamma-2 |
| Molecular Weight(MW) | 150 kDa |
| Tissue Specificity | This antibody detects endogenous level of total PLCγ2 protein. |
| Entrez Gene | 5336 |
|---|---|
| Protein Accession | NP_002652.2 |
Application
Immunofluorescence staining of methanol-fixed HeLa cells using PLCγ2 (Ab-753).
Application
Western blot analysis of lysed extracts from 293 and 3T3 cells using PLCγ2 (Ab-753).| Application Notes | Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
