Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using PLD2 (Phospho-Tyr169) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from Jurkat cells treated with TNF using PLD2 (Phospho-Tyr169) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | PLD2 (phospho Tyr169) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PLD 2, PLD1C, choline phosphatase 2 |
| Product description | Phosphatidylcholine (PC)-specific phospholipases D (PLDs) catalyze the hydrolysis of PC to produce phosphatidic acid and choline. Activation of PC-specific PLDs occurs as a consequence of agonist stimulation of both tyrosine kinase and G protein-coupled receptors. PC-specific PLDs have been proposed to function in regulated secretion, cytoskeletal reorganization, transcriptional regulation, and cell cycle control.1) Steed P.M., FASEB J. 12:1309-1317(1998). |
| Immunogen | PLD2 (phospho Tyr169) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 169 (E-N-Y (p)-L-N) derived from Human PLD2. |
| Modification | p-Tyr169 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PLD2 |
|---|---|
| Alternative Names | PLD 2 PLD1C choline phosphatase 2 |
| Molecular Weight(MW) | 95 kDa |
| Tissue Specificity | The antibody detects endogenous levels of PLD2 only when phosphorylated at tyrosine 169. |
| Entrez Gene | 5338 |
|---|---|
| Protein Accession | NP_002654.3 |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using PLD2 (Phospho-Tyr169) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from Jurkat cells treated with TNF using PLD2 (Phospho-Tyr169) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
