Application
Western blot analysis of PNKP using rabbit polyclonal PNKP Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PNKP gene.Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).| Product Name | PNKP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Bifunctional polynucleotide phosphatase/kinase, DNA 5'-kinase/3'-phosphatase, Polynucleotide kinase-3'-phosphatase, Polynucleotide 3'-phosphatase, 2'(3')-polynucleotidase, Polynucleotide 5'-hydroxyl-kinase, PNKP |
| Product description | This locus represents a gene involved in DNA repair. In response to ionizing radiation or oxidative damage, the protein encoded by this locus catalyzes 5' phosphorylation and 3' dephosphorylation of nucleic acids. Mutations at this locus have been associated with microcephaly, seizures, and developmental delay.1) Arora, M., et al. Leukemia 24(8):1470-1475(2010) |
| Immunogen | This PNKP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-138 amino acids from the N-terminal region of human PNKP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PNKP |
|---|---|
| Alternative Names | Bifunctional polynucleotide phosphatase/kinase DNA 5'-kinase/3'-phosphatase Polynucleotide kinase-3'-phosphatase Polynucleotide 3'-phosphatase 2'(3')-polynucleotidase Polynucleotide 5'-hydroxyl-kinase PNKP |
| Molecular Weight(MW) | 57 kDa |
Application
Western blot analysis of PNKP using rabbit polyclonal PNKP Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PNKP gene.
Application
Western blot analysis in Jurkat cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
