Application
Antibody used in IHC on Human kidney at 5.0 ug/ml.Application
Antibody used in WB on Human Jurkat 0.5 ug/ml.| Product Name | PODXL Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PODXL, Gp200, MGC138240, PCLP, PC, PCLP-1 |
| Product description | PODXL is a member of the sialomucin protein family. PODXL was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin.This gene encodes a member of the sialomucin protein family. The encoded protein was originally identified as an important component of glomerular podocytes. Podocytes are highly differentiated epithelial cells with interdigitating foot processes covering the outer aspect of the glomerular basement membrane. Other biological activities of the encoded protein include: binding in a membrane protein complex with Na+/H+ exchanger regulatory factor to intracellular cytoskeletal elements, playing a role in hematopoetic cell differentiation, and being expressed in vascular endothelium cells and binding to L-selectin.1) Qiu, D., (2008) Biochem. Biophys. Res. Commun. 369 (2), 735-740. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PODXL. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PODXL antibody can be used for detection of PODXL by ELISA at 1:312500. PODXL antibody can be used for detection of PODXL by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PODXL |
|---|---|
| Alternative Names | PODXL Gp200 MGC138240 PCLP PC PCLP-1 |
| Molecular Weight(MW) | 56 kDa |
| Entrez Gene | 5420 |
|---|---|
| Protein Accession | NP_001018121 |
Application
Antibody used in IHC on Human kidney at 5.0 ug/ml.
Application
Antibody used in WB on Human Jurkat 0.5 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | PODXL antibody can be used for detection of PODXL by ELISA at 1:312500. PODXL antibody can be used for detection of PODXL by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PODXL antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
