POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.1) Bolognese, F., et al., (2000) Nucleic Acids Res. 28 (19), 3830-3838.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLE3.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
POLE3 antibody can be used for detection of POLE3 by ELISA at 1:312500. POLE3 antibody can be used for detection of POLE3 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
POLE3 antibody can be used for detection of POLE3 by ELISA at 1:312500. POLE3 antibody can be used for detection of POLE3 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store POLE3 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt