POLL is a repair polymerase.It is involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA. Has both DNA polymerase and terminal transferase activities. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity.1) Picher, A.J. (2007) DNA Repair (Amst.) 6 (12), 1749-1756.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLL.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
POLL antibody can be used for detection of POLL by ELISA at 1:12500. POLL antibody can be used for detection of POLL by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Brain at 0.2-1 ug/ml.
Positive Control
1) Cat. No. XBL-10123 - Fetal Brain Tissue Lysate
Application Notes
POLL antibody can be used for detection of POLL by ELISA at 1:12500. POLL antibody can be used for detection of POLL by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store POLL antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.