Application
Antibody used in WB on Human Heart at 0.2-1 ug/ml.| Product Name | POLR3B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | POLR3B belongs to the RNA polymerase beta chain family. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3B is the second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It is proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft.1) Yee, N.S., PLoS Biol. 5 (11), E312 (2007). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLR3B. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| POLR3B antibody can be used for detection of POLR3B by ELISA at 1:12500. POLR3B antibody can be used for detection of POLR3B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | DrosophilaHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | POLR3B |
|---|---|
| Alternative Names | POLR3B FLJ10388 RPC2 C128 HLD8 |
| Molecular Weight(MW) | 128 kDa |
Application
Antibody used in WB on Human Heart at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10407 - Fetal Heart Tissue Lysate |
|---|---|
| Application Notes | POLR3B antibody can be used for detection of POLR3B by ELISA at 1:12500. POLR3B antibody can be used for detection of POLR3B by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store POLR3B antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
