Application
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.Application
Antibody used in WB on Human 293T at 1 ug/ml.| Product Name | POLR3F Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | POLR3F is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase.The protein encoded by this gene is one of more than a dozen subunits forming eukaryotic RNA polymerase III (RNA Pol III), which transcribes 5S ribosomal RNA and tRNA genes. This protein has been shown to bind both TFIIIB90 and TBP, two subunits of RNA polymerase III transcription initiation factor IIIB (TFIIIB). Unlike most of the other RNA Pol III subunits, the encoded protein is unique to this polymerase.1) Stelzl, U., (2005) Cell 122 (6), 957-968. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POLR3F. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| POLR3F antibody can be used for detection of POLR3F by ELISA at 1:62500. POLR3F antibody can be used for detection of POLR3F by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | POLR3F |
|---|---|
| Alternative Names | POLR3F MGC13517 RPC39 RPC6 |
| Molecular Weight(MW) | 36 kDa |
Application
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.
Application
Antibody used in WB on Human 293T at 1 ug/ml.| Positive Control | 1) 721_B Cell Lysate |
|---|---|
| Application Notes | POLR3F antibody can be used for detection of POLR3F by ELISA at 1:62500. POLR3F antibody can be used for detection of POLR3F by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store POLR3F antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
