The POU6F1 gene encodes a protein that is part of a family of transcription factors which exhibit distinct temporal and spatial patterns of expression.1) Wey, E., (1994) Eur. J. Biochem. 220 (3), 753-762.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human POU6F1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
POU6F1 antibody can be used for detection of POU6F1 by ELISA at 1:62500. POU6F1 antibody can be used for detection of POU6F1 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
POU6F1 antibody can be used for detection of POU6F1 by ELISA at 1:62500. POU6F1 antibody can be used for detection of POU6F1 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store POU6F1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.