Application
Antibody used in WB on Human Jurkat 2.5 ug/ml.Application
Antibody used in IHC on Mouse at 125 ug/ml.| Product Name | PPAP2A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PPAP2A is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is an integral membrane glycoprotein, and has been shown to be a surface enzyme that plays an active role in the hydrolysis and uptake of lipids from extracellular space. The expression of PPAP2A is found to be regulated by androgen in a prostatic adenocarcinoma cell line.The protein encoded by this gene is a member of the phosphatidic acid phosphatase (PAP) family. PAPs convert phosphatidic acid to diacylglycerol, and function in de novo synthesis of glycerolipids as well as in receptor-activated signal transduction mediated by phospholipase D. This protein is an integral membrane glycoprotein, and has been shown to be a surface enzyme that plays an active role in the hydrolysis and uptake of lipids from extracellular space. The expression of this gene is found to be regulated by androgen in a prostatic adenocarcinoma cell line. At least two alternatively spliced transcript variants encoding distinct isoforms have been described.1) Tanyi, J.L., Clin. Cancer Res. 9 (10 PT 1), 3534-3545 (2003). |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PPAP2A. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PPAP2A antibody can be used for detection of PPAP2A by ELISA at 1:62500. PPAP2A antibody can be used for detection of PPAP2A by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | HumanMouseZebra Fish |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PPAP2A |
|---|---|
| Alternative Names | PPAP2A LLP1a LPP1 PAP-2a PAP2 PAP2a2 PAP2alpha2 PAPalpha1 |
| Molecular Weight(MW) | 31 kDa, 32 kDa |
Application
Antibody used in WB on Human Jurkat 2.5 ug/ml.
Application
Antibody used in IHC on Mouse at 125 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | PPAP2A antibody can be used for detection of PPAP2A by ELISA at 1:62500. PPAP2A antibody can be used for detection of PPAP2A by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PPAP2A antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
Data sheet for OM288571