Application
Western blot analysis in mouse liver tissue lysates (35ug/lane).Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Product Name | PPARA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined.1) Jablonski, K.A., et al. Diabetes 59(10):2672-2681(2010) |
| Immunogen | This PPARA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-181 amino acids from the Central region of human PPARA. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PPARA |
|---|---|
| Alternative Names | Peroxisome proliferator-activated receptor alpha PPAR-alpha Nuclear receptor subfamily 1 group C member 1 PPARA NR1C1 PPAR |
| Molecular Weight(MW) | 52 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western blot analysis in mouse liver tissue lysates (35ug/lane).
Application
Flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.| Application Notes | For WB starting dilution is: 1:1000 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
