Application
Western blot analysis of PPID using rabbit polyclonal PPID Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPID gene.Application
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PPID Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of PPID Antibody in NIH-3T3 cell line lysates (35ug/lane)| Product Name | PPID Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.1) Mayya V.,et.al., Sci. Signal. 2:RA46-RA46(2009). |
| Immunogen | This PPID antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-115 amino acids from the N-terminal region of human PPID. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | PPID |
|---|---|
| Alternative Names | Peptidyl-prolyl cis-trans isomerase D PPIase D 40 kDa peptidyl-prolyl cis-trans isomerase Cyclophilin-40 CYP-40 Cyclophilin-related protein Rotamase D PPID CYP40 CYPD |
| Molecular Weight(MW) | 41 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western blot analysis of PPID using rabbit polyclonal PPID Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPID gene.
Application
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PPID Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of PPID Antibody in NIH-3T3 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
