Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).| Product Name | PPIF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PPIF is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death.1) Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) : |
| Immunogen | This PPIF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-131 amino acids from the Central region of human PPIF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PPIF |
|---|---|
| Alternative Names | Peptidyl-prolyl cis-trans isomerase F mitochondrial PPIase F Cyclophilin D CyP-D CypD Cyclophilin F Mitochondrial cyclophilin CyP-M Rotamase F PPIF CYP3 |
| Molecular Weight(MW) | 22 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
