Application
PPP6C Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of anti-PPP6C Antibody in Jurkat cell line lysates (35ug/lane).| Product Name | PPP6C Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PPP6C belongs to the PPP phosphatase family, PP-V subfamily. Reversible phosphorylation of proteins on serine and threonine residues is an important biochemical event that regulates a broad variety of intracellular processes. The phosphorylation state is determined by the well-controlled balance of activities of serine/threonine-specific protein kinases and protein phosphatases, including PPP6C. Expression levels are highest in testis, heart, and skeletal muscle and lowest in placenta, lung, and kidney. PPP6C can complement mutations in the S. cerevisiae Sit4 and S. pombe ppe1 genes, indicating that PPP6C is the functional homolog of yeast Sit4p and ppe1. Since Sit4p is required for the G1 to S transition of the cell cycle and ppe1 is involved in cell shape control and mitotic division, it has been suggested that PPP6C functions in cell cycle regulation.1) Yang, J., et al., EMBO J. 24(1):1-10 (2005). |
| Immunogen | This PPP6C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-305 amino acids from the C-terminal region of human PPP6C. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | PPP6C |
|---|---|
| Alternative Names | Serine/threonine-protein phosphatase 6 catalytic subunit PP6C Serine/threonine-protein phosphatase 6 catalytic subunit N-terminally processed PPP6C PPP6 |
| Molecular Weight(MW) | 35 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
PPP6C Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of anti-PPP6C Antibody in Jurkat cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
